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Enzyme and Microbial Biochemistry Lab
Department of Chemistry, IIT Delhi
Extremophiles
&
their enzymes
Our focus lies in the screening, isolation, and characterization of extremophiles, with a special emphasis on halophiles, archaea, and microorganisms with solvent tolerance. In the realm of extremozymes, our research extends to the purification of enzymes and the detailed characterization of their novel genes. This exploration aims to unravel the molecular basis of stability inherent in these enzymes, offering insights into their proteomics.
Selected Publications
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Nitin Srivastava, Sugathan Shiburaj, and S.K. Khare. Improved production of alkaline and solvent-stable proteases from a halotolerant Exiguobacterium isolate through heterologous expression. International Journal of Biological Macromolecules, 260 (1), 129507, 2024
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Nitin Srivastava, Sugathan Shiburaj, and S.K. Khare. Pan-genomic comparison of a potential solvent-tolerant alkaline protease-producing Exiguobacterium sp. TBG-PICH-001 isolated from a marine habitat. 3 Biotech, 13, 371, 2023
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Massimiliano Fenice, S.K. Khare and Susanna Gorrasi. Editorial: Mining, Designing, Mechanisms and Applications of Extremophilic Enzymes. Frontiers in Microbioliogy, 12, 709377, 2021
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Nitin Srivastava, Sumit Kumar, Sugathan Shiburaj, Anshu Gupta and S.K. Khare . Cellular adaptation responses in a halotolerant Exiguobacterium exhibiting organic solvent tolerance with simultaneous protease production, Environmental Technology and Innovation, 23, 101803, 2021
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Ayesha Sadaf, Rajeshwari Sinha , and S. K. Khare "Proteomic profiling of Sporotrichum thermophile under the effect of ionic liquids: manifestation of an oxidative stress response", 3 Biotech, 9, 240, 2019
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